LongAmpTaq2X Master Mix from New England Biolabs

Product Specs
ItemLongAmpTaq2X Master Mix
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0287S
Quantity100 reactions
Concentration2 X
ApplicationsDNA Analysis
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeMaster Mix
Temperature-20 C

Specifications/Features

Information:
Source:
An E. coli strain that carries the Taq? DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent® DNA Polymerase gene from Pyrococcus species GB-D.
Applications:
Long Range PCR

Reagents Supplied:
LongAmp™ Taq Master Mix

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Automated Platform for the Plasmid Construction ProcessACS Synthetic BiologyDecember 15, 2023Alberto A. Nava, Anna Lisa Fear, Namil Lee, Peter Mellinger, Guangxu Lan, Joshua McCauley, Stephen Tan, Nurgul Kaplan, Garima Goyal, R. Cameron Coates, et al.colony PCR validation. For colony PCR validation of the 1018 colonies, we utilized LongAmp Taq (NEB #M0287L) to amplify the full PKS coding sequence (CDS). We observed expected CDS sizes in 417 out of 1018 colonies

(2) Proton Microbeam Targeted Irradiation of the Gonad Primordium Region Induces Developmental Alterations Associated with Heat Shock Responses and Cuticle Defense in Caenorhabditis elegansBiology (Basel)October 27, 2023Pierre Beaudier, Guillaume Devès, Laurent Plawinski, Denis Dupuy, Philippe Barberet, Hervé Seznecreaction mix consisted of 25 µL 2× LongAmp Taq master mix (New England Biolabs, Evry, France, cat. no. M0287S), 1.5 µL barcoded primers (named BP01 to BP12), 18.5 µL nuclease-free water, and 5 µL (~0.25 ng) cDNA.

(3) Machine learning-based detection of adventitious microbes in T-cell therapy cultures using long-read sequencingMicrobiology SpectrumOctober 17, 2023James P. B. Strutt, Meenubharathi Natarajan, Elizabeth Lee, Denise Bei Lin Teo, Wei-Xiang Sin, Ka-Wai Cheung, Marvin Chew, Khaing Thazin, Paul W. Barone, Jacqueline M. Wolfrum, et al.65°C for 29 cycles, followed by a final 5 min extension at 65°C, LongAmp Taq 2X Master Mix (e.g., NEB M0287). Long-read sequencing library preparation We used a multiplexing approach to maximize the sample

(4) Accuracy of Nanopore Sequencing as a Diagnostic Assay for Pulmonary Tuberculosis versus Smear, Culture and Xpert MTB/RIF: A Head-to-Head ComparisonTropical Medicine and Infectious DiseaseSeptember 8, 2023Juan Yang, Wei Ye, Chao Zhang, Wenhong Lin, Lin Mei, Shengsheng Liu, Jie Liuassay was prepared according to the instructions included with the LongAmp Taq 2× Master Mix Kit (#M0287, NEB, Illumina, San Diego, CA, USA). The initial PCR cycling step (94 °C for 2 min) was followed by

(5) No Clear Clustering Dysbiosis from Salivary Microbiota Analysis by Long Sequencing Reads in Patients Affected by Oral Squamous Cell Carcinoma: A Single Center StudyCancers (Basel)August 22, 2023Rodolfo Mauceri, Martina Coppini, Davide Vacca, Giorgio Bertolazzi, Valeria Cancila, Claudio Tripodo, Giuseppina Campisiincluded 1 μL of Rapid Barcode Primer at 10 µM, 25 μL of LongAmp® Hot Start Taq 2× Master Mix (NEB cat No. M0287), and Nuclease-free water, up to a final volume of 50 µL. PCR profile included an initial denaturation

(6) Benchmarking assembly free nanopore read mappers to classify complex millipede gut microbiota via Oxford Nanopore Sequencing TechnologyJournal of Biological MethodsAugust 4, 2023Orlando J. Geli-Cruz, Carlos J. Santos-Flores, Matias J. Cafaro, Alexander Ropelewski, Alex R. Van DamTechnologies), 2 μL of 10 ng/μL adapter ligated template, 50 μL of NEB LongAmp Taq 2X Master Mix (NEB Cat. #M0287, New England Biolabs), and 46 μL of nuclease-free water were mixed. Ligation of Sequencing Adapter 20

(7) Condensate cooperativity underlies transgenerational gene silencingCell ReportsAugust 1, 2023Zhenzhen Zhenzhen, Kun Shi, Jordan S. Brown, Tao He, Wei-Sheng Wei-Sheng, Ying Zhang, Heng-Chi Heng-Chi, Donglei ZhangDithiothreitol (DTT),Sangon Bioteh,Cat#A620058; CAS:3483–12-3 Long AMP Taq 2X Master Mix,New England Biolabs,Cat#M0287S 2X Taq Plus Master Mix II,Vazyme,Cat#P213–01 Phanta Max Super-Fidelity DNA polymerase,Vazyme,Cat#P505-d1 ,, Critical

(8) Transcript capture and ultradeep long-read RNA sequencing (CAPLRseq) to diagnose HNPCC/Lynch syndromeJournal of Medical GeneticsAugust 1, 2023Vincent Schwenk, Rafaela Magalhaes Leal Silva, Florentine Scharf, Katharina Knaust, Martin Wendlandt, Tanja Häusser, Julia M A Pickl, Verena Steinke-Lange, Andreas Laner, Monika Morak, et al.pairs (online supplemental table 1) using LongAmp Taq Master Mix (New England Biolabs, catalogue number M0287) with the following cycling conditions: denaturation at 95°C for 30 s, 14 cycles of 95°C for 15 s, 62°C

(9) Increased prime edit rates in KCNQ2 and SCN1A via single nicking all-in-one plasmidsBMC BiologyJuly 13, 2023N. Dirkx, Wout J. Weuring, E. De Vriendt, N. Smal, J. van de Vondervoort, Ruben van ’t Slot, M. Koetsier, N. Zonnekein, Tim De Pooter, S. Weckhuysen, et al.barcoding (see Additional file 1: Fig. S8). Barcoding was done using the LongAmp Taq 2X Master Mix (M0287L, New England Biolabs) and PCR Barcoding Expansion 1–96 kit (EXP-PBC096, Oxford Nanopore Technologies)

(10) A Cas3-base editing tool for targetable in vivo mutagenesisNature CommunicationsJune 9, 2023Anna Zimmermann, Julian E. Prieto-Vivas, Charlotte Cautereels, Anton Gorkovskiy, Jan Steensels, Yves Van de Peer, Kevin J. VerstrepenExpansion 1-96 kit (EXP-PBC096, Oxford Nanopore Technologies (ONT)) and the LongAmp Taq 2X Master Mix (M0287L, New England Biolabs). Barcoded samples/amplicons were purified using AMPure XP reagent (Beckman Coulter),

(11) Cochlear transcript diversity and its role in auditory functions implied by an otoferlin short isoformNature communicationsMay 29, 2023Liu H, Liu H, Wang L, Song L, Jiang G, Lu Q, Yang T, Peng H, Cai R, Zhao X, et al.Thermo Fisher Scientific, USA) and then amplified using suitable PCR cycles with using LongAmp Taq 2X Master Mix (CAT#M0287, NEB). Damage repair and end repair were carried out before cDNA was ligated to sequencing adapters

(12) Rapid viral metagenomics using SMART-9N amplification and nanopore sequencingWellcome Open ResearchApril 24, 2023Ingra M. Claro, Mariana S. Ramundo, Thais M. Coletti, Camila A. M. da Silva, Ian N. Valenca, Darlan S. Candido, Flavia C. S. Sales, Erika R. Manuli, Jaqueline G. de Jesus, Anderson de Paula, et al.70°C. These double-tagged cDNA products were amplified using 25 μl LongAmp Taq 2X master mix (Cat No. M0287, New England BioLabs, USA), 19.5 μl of NFW, 0.5 μl RLB (1-12) from SQK-RPB004 kit (ONT, UK) and 5 μl

(13) Multicellular, IVT-derived, unmodified human transcriptome for nanopore direct RNA analysisbioRxivApril 13, 2023Caroline A. McCormick, Stuart Akeson, Sepideh Tavakoli, Dylan Bloch, Isabel N. Klink, Miten Jain, Sara H. Rouhanifard(1 cycle) and hold at 4°C (∞). PCR amplification was performed using LongAmp Taq 2X Master Mix (NEB, M0287S) and the Nanopore_T7_IVT_Forward and Reverse primers. The thermocycling conditions are as follows: initial

(14) Polycomb response elements reduce leaky expression of Cas9 under temperature-inducible Hsp70Bb promoter in Drosophila melanogasterG3 Genes|Genomes|GeneticsApril 11, 2023Natalie Warsinger-Pepe, Carly Chang, Connor R Desroberts, Omar S AkbariPCR amplification. PCR amplification of all target regions was performed using LongAmp Taq 2X Mastermix (NEB #M0287). Amplicon size was verified using gel electrophoresis, and amplicons were purified using QIAquick PCR

(15) An Efficient Method to Prepare Barcoded cDNA Libraries from Plant Callus for Long-Read SequencingMethods and ProtocolsMarch 15, 2023Daniela Cordeiro, Alexandra Camelo, Ana Carolina Pedrosa, Inês Brandão, Jorge Canhoto, Christophe Espírito Santo, Sandra CorreiaCat. No. 51976)Flow Cell R9.4.1 (ONT, Cat. No. FLO-MIN106)LongAmp® Taq 2X Master Mix (NEB, Cat. No. M0287)Maxima H Minus Reverse Transcriptase (Thermo Scientific™, ™, Thermo Fisher Scientific, Massachusetts,

(16) Diagnostic value of a nanopore sequencing assay of bronchoalveolar lavage fluid in pulmonary tuberculosisBMC Pulmonary MedicineMarch 8, 2023Zhifeng Liu, Yang Yang, Qingfeng Wang, Lei Wang, Wenjuan Nie, Naihui Chuprepared the PCR mix as directed by the instructions provided with the LongAmp Taq 2 × Master Mix Kit (#M0287, NEB). The 30-μL PCR reaction mix for each sample included 5 μL each of 10 μM forward and reverse primers

(17) Brome mosaic virus detected in Kansas wheat co-infected with other common wheat virusesFrontiers in Plant ScienceMarch 3, 2023Nar B. Ranabhat, John P. Fellers, Myron A. Bruce, Jessica L. Shoup Ruppsamples, 18.5 µl of NFW, and 25 µl of 2X LongAmp Taq Master Mix (New England Biolabs, catalog number #M0287). PCR amplification consisted of 95°C for 30secs for initial denaturation, 15 cycles of 95°C for 15secs,

(18) Semi-quantitative detection of pseudouridine modifications and type I/II hypermodifications in human mRNAs using direct long-read sequencingNature CommunicationsJanuary 19, 2023Sepideh Tavakoli, Mohammad Nabizadeh, Amr Makhamreh, Howard Gamper, Caroline A. McCormick, Neda K. Rezapour, Ya-Ming Hou, Meni Wanunu, Sara H. Rouhanifardand reverse primers were added to the product and PCR amplified using LongAmp Taq 2X Master Mix (NEB, M0287S) with the following cycling conditions: initial denaturation 30 s @ 95 °C (1 cycle), denaturation 15

(19) NAb-seq: an accurate, rapid, and cost-effective method for antibody long-read sequencing in hybridoma cell lines and single B cellsmAbsDecember 31, 2022Hema Preethi Subas Satish, Kathleen Zeglinski, Rachel T. Uren, Stephen L. Nutt, Matthew E. Ritchie, Quentin Gouil, Ruth M. Kluckbarcoding using 12 cycles of PCR. Each PCR reaction mix consisted of 25 µl 2x LongAmp Taq master mix (M0287; New England Biolabs), 1.5 µl barcoded primers A or B (for ease, barcodes BP01 and BP02 from the kit

(20) SCON-a Short Conditional intrON for conditional knockout with one-step zygote injectionExperimental & molecular medicineDecember 1, 2022Wu SS, Lee H, Szép-Bakonyi R, Colozza G, Boese A, Gert KR, Hallay N, Lee JH, Kim J, Zhu Y, et al.clear part of the solution was used for PCR with either Gotaq (Promega, M7808) or LongAmp 2X (NEB, M0287S) according to the manufacturer’s instructions. To check the sequences of genomic DNA, PCR bands of the

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